WASHINGTON, Feb 25 (Reuters) - The top Democrat on the U.S. Senate Commerce Committee on Wednesday urged the House of Representatives to take a new vote on aviation legislation that failed by one vote after opposition by the Pentagon.

The House voted 264-133 in favor of the ROTOR Act, passed unanimously by the Senate in December, to address concerns after an American Airlines AAL.O regional jet and an Army Black Hawk helicopter collided in the crowded airspace over the nation's capital, killing 67 people. Senator Maria Cantwell called on the House to take a new vote.

Under the fast-track rules, the bill needed a two-thirds majority to pass -- and it fell one vote short.

(Reporting by David Shepardson; Editing by Chizu Nomiyama )

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